An HRP-labeled lateral flow immunoassay for rapid simultaneous detection and differentiation of influenza A and B viruses.
Identifieur interne : 000917 ( Main/Exploration ); précédent : 000916; suivant : 000918An HRP-labeled lateral flow immunoassay for rapid simultaneous detection and differentiation of influenza A and B viruses.
Auteurs : Jing Zhang [République populaire de Chine] ; Xun Gui [République populaire de Chine] ; Qingbing Zheng [République populaire de Chine] ; Yixin Chen [République populaire de Chine] ; Shengxiang Ge [République populaire de Chine] ; Jun Zhang [République populaire de Chine] ; Ningshao Xia [République populaire de Chine]Source :
- Journal of medical virology [ 1096-9071 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine.
- Bandelettes réactives, Dosage immunologique, Horseradish peroxidase, Humains, Limite de détection, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Horseradish Peroxidase, Reagent Strips.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A virus, Influenza B virus.
- methods : Immunoassay.
- virology : Influenza, Human.
- Humans, Limit of Detection, Sensitivity and Specificity.
Abstract
Rapid and sensitive diagnosis of influenza is urgently needed to address the limitations of low sensitivity associated with current rapid tests available for clinics and on-site monitoring. A novel horseradish peroxidase (HRP)-labeled lateral flow immunoassay strip (HRP-LFIA) for rapid simultaneous detection and differentiation of influenza A (INF A) and influenza B (INF B) viruses were developed. This immunoassay was based on the signal amplification by the HRP-catalyzed oxidation of 3, 3', 5, 5'-tetramethylbenzidine forming a colored insoluble product, which was proportional to the analyte concentration. Compared with conventional gold-colloidal based strips, an analytical sensitivity enhancement of more than one order of magnitude for thirteen INF virus isolates was observed. A total of 1487 swabs obtained from persons with influenza-like illnesses were tested for the presence of INF A and B viruses using real-time reverse transcription polymerase chain reaction (rRT-PCR) as the reference criterion. The overall sensitivities of HRP-LFIA were 77.5% (100/129) and 71.2% (116/163) for INF A and INF B, respectively. The overall specificities were 99.8% (1144/1146) and 99.8% (918/920), respectively. The nasopharyngeal sampling method yielded higher sensitivity rates of 90.2% (55/61) and 82.6% (71/86). In conclusion, this user-friendly assay could be a promising rapid detection method for rapid screening of INF A and INF B viruses.
DOI: 10.1002/jmv.25322
PubMed: 30238471
Affiliations:
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Le document en format XML
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Xiamen</wicri:regionArea><wicri:noRegion>Xiamen</wicri:noRegion></affiliation></author><author><name sortKey="Gui, Xun" sort="Gui, Xun" uniqKey="Gui X" first="Xun" last="Gui">Xun Gui</name><affiliation wicri:level="1"><nlm:affiliation>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen</wicri:regionArea><wicri:noRegion>Xiamen</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Qingbing" sort="Zheng, Qingbing" uniqKey="Zheng Q" first="Qingbing" last="Zheng">Qingbing Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen</wicri:regionArea><wicri:noRegion>Xiamen</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, 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Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen</wicri:regionArea><wicri:noRegion>Xiamen</wicri:noRegion></affiliation></author><author><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><affiliation wicri:level="1"><nlm:affiliation>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Experimental Medicine, State Key Laboratory of Molecular Vaccine and Molecular Diagnostics, School of Public Health, Xiamen University, 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peroxidase ()</term><term>Humains</term><term>Limite de détection</term><term>Sensibilité et spécificité</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus influenza B (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Horseradish Peroxidase</term><term>Reagent Strips</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Limit of Detection</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bandelettes réactives</term><term>Dosage immunologique</term><term>Horseradish peroxidase</term><term>Humains</term><term>Limite de détection</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rapid and sensitive diagnosis of influenza is urgently needed to address the limitations of low sensitivity associated with current rapid tests available for clinics and on-site monitoring. A novel horseradish peroxidase (HRP)-labeled lateral flow immunoassay strip (HRP-LFIA) for rapid simultaneous detection and differentiation of influenza A (INF A) and influenza B (INF B) viruses were developed. This immunoassay was based on the signal amplification by the HRP-catalyzed oxidation of 3, 3', 5, 5'-tetramethylbenzidine forming a colored insoluble product, which was proportional to the analyte concentration. Compared with conventional gold-colloidal based strips, an analytical sensitivity enhancement of more than one order of magnitude for thirteen INF virus isolates was observed. A total of 1487 swabs obtained from persons with influenza-like illnesses were tested for the presence of INF A and B viruses using real-time reverse transcription polymerase chain reaction (rRT-PCR) as the reference criterion. The overall sensitivities of HRP-LFIA were 77.5% (100/129) and 71.2% (116/163) for INF A and INF B, respectively. The overall specificities were 99.8% (1144/1146) and 99.8% (918/920), respectively. The nasopharyngeal sampling method yielded higher sensitivity rates of 90.2% (55/61) and 82.6% (71/86). In conclusion, this user-friendly assay could be a promising rapid detection method for rapid screening of INF A and INF B viruses.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name></noRegion><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><name sortKey="Ge, Shengxiang" sort="Ge, Shengxiang" uniqKey="Ge S" first="Shengxiang" last="Ge">Shengxiang Ge</name><name sortKey="Gui, Xun" sort="Gui, Xun" uniqKey="Gui X" first="Xun" last="Gui">Xun Gui</name><name sortKey="Xia, Ningshao" sort="Xia, Ningshao" uniqKey="Xia N" first="Ningshao" last="Xia">Ningshao Xia</name><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name><name sortKey="Zheng, Qingbing" sort="Zheng, Qingbing" uniqKey="Zheng Q" first="Qingbing" last="Zheng">Qingbing Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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